Xi'an Prius Biological Engineering Co.,Ltd
Address:Room 0836,Euro-Asia International Building A,Ouya avenue ,Xi'an,Shaanxi Province ,China.
Extraction methods of ginsenosides:
Ultrasonic water extraction：
Weigh 40g of ginseng powder, Add quantitative water to soak overnight, ultrasonic extraction at room temperature for a total of 3 times. Ultrasonic time is 40 min each time. Filter by suction, combine the filtrate, and apply to D101 macroporous adsorption resin column to remove impurities. The water is eluted until the effluent is colorless. Elute with 80% ethanol until the eluent TLC checks for no saponin reaction, The ethanol eluate is recovered under reduced pressure to dryness, the residue is the crude ginsenoside, which is dilute to a 200 ml volumetric flask and shaken for later use.
Wet Pressing Extraction Method：
Weigh 40 g of ginseng powder, dissolve the ginseng powder with 60% ethanol and 70% ethanol respectively, pulverize and extract twice at 1000 r•min -1 at a high speed. After crushing, the medicinal solution is centrifuged at 5000 r•min -1 for 10 min. The clear solution, combine the two extracts, dilute to a 200 ml volumetric flask, shake well and set aside.
Weigh 40 g of ginseng powder and place it in a 500 ml round-bottomed flask. Add 100 ml of 50% methanol solution, extract at reflux for 4 hours, filter with a microporous membrane (0.45 um), and transfer the filtrate to 50% methanol. Fill a 200ml volumetric flask and dilute to the mark. Take an appropriate amount of the filtrate, centrifuge at 1,000 r•min-1 for 10 min, take the supernatant and dilute to a 200 ml volumetric flask, shake well for later use.
Determination of paste rate：
Accurately measure 20 ml from each portion of ginsenoside extract, place it in an evaporating dish, first evaporate to dryness in a water bath, and then dry at 100°C for 3 hours. Take it out and place it in a drying oven for 30 minutes and then take it out and weigh it, calculating the paste rate.
The preparation of the reference substance solution calculates the cream rate:
Accurately weigh the appropriate amounts of ginsenoside Re and Rb1 reference substance, add methanol to make each 1 ml of ginsenoside 0.41mg, shake well, and use as a reference substance stock solution.
Preparation of test solution:
Precisely weigh 10 ml of the above-mentioned ginsenoside extracts, place them in an evaporating dish, and evaporate to dryness in a water bath. Dissolve the residue with an appropriate amount of methanol and dilute to a 10 ml volumetric flask with methanol. Shake well and filter with a microporous membrane to obtain test solution. Separately draw 20 ul of the test solution and the reference solution into the liquid chromatograph, and calculate the extraction rate of ginsenosides obtained by each extraction method.
The extraction rates of ginsenoside Re by reflux extraction method, ultrasonic water extraction method, wet method, and wet crushing extraction method were 83.38%, 90.23%, 96.78%, respectively; the extraction rate of ginsenoside Rb1 was 73.34%, respectively , 80.11%, 90.71%.
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