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Progress in the synthesis of Panax Notoginseng Saponins powder

Nov 05, 2020

Panax notoginseng belongs to the genus Araliaceae ginseng , it is a traditional Chinese herbal medicine in China , which is also one of the most important bulk medicinal materials in Yunnan.

Panax notoginseng saponins.jpgThe main active ingredient is Panax notoginseng saponins, which has good anti-inflammatory, anti-tumor, anti-atherosclerosis, blood pressure and blood sugar lowering pharmacological activities. The content of saponins in the roots of Panax notoginseng is relatively low, it usually takes three years or more of planting time to accumulate. However, the molecular mechanism that the accumulation of saponins increases with the increase in growth years is not clear; moreover, the current understanding of notoginsenoside synthase genes is also less, which greatly limits the research on the biosynthesis of notoginsenosides.

The functional genomics and utilization team of the Kunming Institute of Botany, Chinese Academy of Sciences, Wu Jinsong's research team, and the Zhu Shusheng team of Yunnan Agricultural University, used analytical chemistry, transcriptomics and molecular biology to analyze the characteristics of the synthesis of total saponins in one-year and three-year-olds. The research results show that the content of notoginseng saponins R1, Rb1, and Rg1 in three-year-old roots is higher, which is more than eight times the content in one-year roots; Correspondingly, the expression levels of saponin synthase genes squalene oxidase, dammarene diol synthase and P450 monooxygenase (CYP716A53V2-like) were significantly increased in the three-year-old Panax notoginseng. At the same time, the transcriptome data also showed that the primary product synthesis and metabolism ability of three-year-old Panax notoginseng decreased, while the secondary product synthesis and metabolism ability increased. Finally, the study also cloned the possible synthase genes of dammarenediol and protopanaxadiol in Panax notoginseng, verified their functions through gene transformation, and obtained high-yield dammarenediol and protopanaxadiol Glycol cultivated tobacco.

The study initially revealed the molecular mechanism of the higher content of saponins in three-year-old Panax notoginseng, laying the foundation for the next step of bioengineering synthesis of high-yield Panax notoginseng saponins.

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