Xi'an Prius Biological Engineering Co.,Ltd
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The first observed in vivo effect of copper peptide raw material was to accelerate wound healing in various animal models, using rats for a test in which full-thickness wounds with a diameter of 6 mm were created within an ischaemic double pedicled flap on the back of each rat, and the rats were divided into three groups, namely topical TCC (GHK-Cu), topical TCC vehicle (hydroxypropyl methylcellulose) and no treatment (control), for 13 days with daily measurement of wound area (see Figure 3), Showed that wounds in the TCC group starting on the third day were all significantly smaller than those in the control group. On day 13, photographs of the double pedicled flap (see Fig.4) comparing 6 open wounds on the back of control (upper), vehicle (middle), and TCC (lower) rats show that the wounds healed faster in the TCC group and delayed in the control rats. The results showed that treatment with topical GHK-Cu accelerated the healing of ischemic open wounds.
Figure 3: median wound area (mm2) in each group from day 0 to day 13.TCC GHK-Cu cuprizone; Vehicle vehicle; Control control group.*Indicated that the wounds in the TCC group were significantly smaller than the number of days in the control group (P < 0.05).‡ indicates that wounds in the TCC group were significantly smaller than the number of days with vehicle (P < 0.05).
Figure 4: photographs of a double pedicled flap from 6 open wounds on the back of rats in the control group (top), the TCC vehicle group (middle), and the TCC group (bottom) on day 13.
The later experiment confirmed that copper peptide raw material plays a physiological role in the process of wound healing and tissue repair by stimulating collagen and glycosaminoglycans in fibroblasts. Biochemical analysis was performed after the rats were injected with 2.0 mg GHK Cu or phosphate-buffered saline control, using hydroxyproline and uronic acid as indicators of collagen and GAG content, respectively. The results showed that throughout the experiment, injection of GHK Cu significantly increased the collagen content compared to control on days 18 and 22 (396% vs 538% of control, P < 0.05) (see Fig.5A); Gag accumulation was significantly higher on days 12, 18, and 22 (191%, 180%, and 179%, respectively, of the corresponding control, P < 0.05) (Figure 5B). The results showed that copper peptide raw material could increase the synthesis of skin collagen and glycosaminoglycans.
Figure 5: GHK Cu increased the accumulation of collagen and gags in the wound cavity. Injected twice weekly with GHK Cu (○) and no GHK Cu (●); Symbols represent individual results from these four rats. Mean values are indicated by horizontal bars.