
Red clover, distributed in Europe, the United States, New Zealand and other places, is traditionally considered a respiratory antispasmodic drug. During the livestock breeding process, people observed the estrogenic effects of red clover varieties. After research, the related effects of red clover isoflavones were discovered, which stimulated the development and sales of red clover plant dietary supplements.
Red clover is rich in isoflavones, flavones and flavonols, among which isoflavones are the functional ingredients that everyone is more concerned about.
Red clover extract is an extract of the perennial herbaceous plant Trifolium genus in the Leguminosae family. The main active ingredients are isoflavones, which have anti-cancer, anti-tumor, regulate human hormone balance, improve osteoporosis, and women's menopausal syndrome. It also has antioxidant and free radical scavenging functions. It is a promising natural medicine, health food and functional food supplement.

|
Item |
Specification |
Result |
|
Physical and Chemical Parameters |
||
|
Total isoflavones(HPLC) |
≥40.0% |
42.30% |
|
Extract solvent |
Alcohol 70% & water |
Conforms |
|
Appearance |
Yellow brown fine powder |
Conforms |
|
Odor&Taste |
Characteristic |
Conforms |
|
Particle size |
95% pass 80mesh |
Complies |
|
Loss on Drying |
≤5.0% |
3.21% |
|
Ash |
≤5.0% |
3.10% |
|
Heavy Metals Total |
≤10ppm |
Conforms |
|
As |
≤2.0ppm |
Conforms |
|
Pb |
≤3.0ppm |
Conforms |
|
Cd |
≤1.0ppm |
Conforms |
|
Hg |
≤1.0ppm |
Conforms |
|
PAH (EP standard) |
Sum PAH 4 < 50ppb |
35.80ppb |
|
Microbiological Tests Total Plate Count(USP) |
≤10,000cfu/g |
Conforms |
|
Total Yeast & Mold(USP) |
≤100CFU/g |
Conforms |
|
Escherichia Coli(USP) |
Negative |
Conforms |
|
Salmonella spp(USP) |
Negative |
Conforms |
|
GMO Status |
Non-GMO |
Conforms |
|
Irradiation(USP) |
Irradiation Free |
Conforms |
|
Conclusion: |
Conform to Standard. |
|

1. Plant estrogen-like action.
Isoflavones such chickpea A and B in Red Clover Extract Powder have estrogen-like properties and can affect hormone levels in the body in both directions. When the body's estrogen levels are low, isoflavones can provide additional estrogenic effects. When the body's estrogen level is high, isoflavones can join with estrogen receptors to inhibit hormone combination, lowering the estrogen level.
2. Antispasmodic action.
Trifolium Anesthetized rabbits were given an oral dose of trifolium pratense extract (flavonoids). It was discovered to be effective in small intestine peristalsis and had a similar effect on intestinal pressure as acetylcholine.
3. Hypolipidemic impact.
Oral dose of 200mg/kg per day for 10 days can lower blood cholesterol from 63.0mg% to 32.5 ~ 48.3 mg%, liver cholesterol from 2.16mg/g to 1.84 ~ 1.85 mg/g, and serum triglyceride from 8.2mg/g to 6.7 ~ 6.9 mg/g, potentially preventing.
4. Anti-arrhythmic effects
injection of Trifolium pratense extract (isoflavones) 3 ~ 5 minutes before aconitine injection can prevent arrhythmia in mice, with a median lethal dose (ED50) of 69mg/kg.

1.Animal Feed
Mainly used as feed to increase lactation in cattle, horses, and sheep.
2. Healthcare Products.
Red Clover Extract Powder is quite famous around the world, and its raw medicinal components or extracts are commonly used to treat climacteric syndrome and premenstrual syndrome in women. For example, Promensil tablets sold in the United States contain 294mg of Trifolium pratense extract, which is comparable to 40mg of four chickpea isoflavones. Trifolium pratense extracts of varying specifications are also commonly utilized as food supplements.
3. Skincare product.
Red axle extract contains isoflavones, which have excellent antioxidant, free radical protection, and scavenging properties. It possesses whitening, anti-ageing, and astringent properties.
Reference
[1].Samman, S., Koh, H. S., Flood, V. M., Blakesmith, S. J., Petocz, P., and Lyons-Wall, P. M. Red clover (Trifolium pratense) isoflavones and serum homocysteine in premenopausal women: a pilot study. J.Womens Health (Larchmt.) 2009;18(11):1813-1816. View abstract.




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