Papaya Extract Papain is a plant protease extracted from papaya pulp. Its active ingredients include cysteine, which is a thiol protease. It has the characteristics of high enzyme activity, good thermal stability, natural safety, etc. It is very effective for animal and plant proteins, Polypeptides, esters, amides, etc. have strong enzymatic hydrolysis ability. Papain is a proteolytic enzyme consisting of a single peptide chain containing 212 amino acid residues.
Its appearance is white to light yellow powder, slightly hygroscopic. Papain powder is soluble in water and glycerin, and the aqueous solution is colorless or light yellow.
COA of Papaya Extract
| Items | Specifications | Results |
| Appearance | White Powder | Conform |
| Odor | Free of offensive odors | Conform |
| Particle size | 98% pass 80 mesh | Conform |
| Activity of enzyme | ≥100,000 U/g | 102,500 U/g |
| Loss on drying | ≤5.0 % | 2.20 % |
| Loss on ignition | ≤5.0 % | 3.00 % |
| Heavy metals | ＜20 mg/kg | ＜20 mg/kg |
| Pb | ＜2.0 mg/kg | ＜2.0 mg/kg |
| As | ＜2.0 mg/kg | ＜2.0 mg/kg |
| Microbiology | ||
| Total Plate Count | <10,000 cfu/g | 680 cfu/g |
| E.Coli | None detected in 10g | Absent |
| Salmonella | None detected in 10g | Absent |
| Yeast and Mould | NMT 100 cfu/g | conform |
| Conclusion: | Comform to specification | |
| Storage | Stored humidity protected (RH less than 60) at temperature below 25℃ | |
1. As a meat tenderizer
Papaya Extract Papain is one of the most popular meat tenderizers. Papain has been used as a "meat tenderiser" for thousands of years. Thousands of years ago, indigenous inhabitants in South America learned that papaya emulsion could be used to tenderize tough meat.
It is employed in the modern food business because of its capacity to break down tough meat fibers, making the flesh more soft.This is because papain contains cysteine protease, which can damage connective tissue and myofibrils.
Furthermore, this enzyme has good thermal stability; even when processed at high temperatures, the enzyme's activity is maintained without altering the nutrition of the meat, making the meat easy to chew and tasty.
2. Papain is a natural plant protease that can help break down debris in the intestines, including proteins, sugars, and fats.
3. Exfoliation
Due to its strong ability to hydrolyze proteins, papain can act on the aging stratum corneum of the skin, promoting its degeneration and removal to achieve skin rejuvenation and promote cell growth. It is also gentler than traditional acid brushing.
4. Whitening effect
Papain can hydrolyze casein into tyrosine, reducing the pigment deposited in the skin and lightening dark brown spots on the skin. And it can block the catalytic effect of tyrosine, thereby reducing the formation of melanin.
As a broad-spectrum enzyme preparation used to improve the nutritional value or functional properties of animal and plant proteins, Papaya Extract Papain powder can be widely used in food, medicine, feed, daily chemicals, leather and textile industries.
Reference
[1].Dos Anjos M.M., da Silva A.A., de Pascoli I.C., Mikcha J.M., Machinski M., Jr., Peralta R.M., de Abreu Filho B.A. Antibacterial activity of papain and bromelain on Alicyclobacillus spp. Int. J. Food Microbiol. 2016;216:121–126. doi: 10.1016/j.ijfoodmicro.2015.10.007.
[2].Stremnitzer C, Manzano-Szalai K, Willensdorfer A, Starkl P, Pieper M, Konig P, Mildner M, Tschachler E, Reichart U, Jensen-Jarolim E: Papain Degrades Tight Junction Proteins of Human Keratinocytes In Vitro and Sensitizes C57BL/6 Mice via the Skin Independent of its Enzymatic Activity or TLR4 Activation. J Invest Dermatol. 2015 Jul;135(7):1790-1800. doi: 10.1038/jid.2015.58. Epub 2015 Feb 23.
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