Xi'an Prius Biological Engineering Co.,Ltd
Address:Room 0836, Euro-Asia International Building A, Ouya avenue, Xi'an, Shaanxi Province, China
Zip code:710038
Phone:+86-17792290970
Mail:sales@prsbiotech.com
Whatsapp:+8617792290970
Tianeptine is a tricyclic antidepressant, which has a good effect on mood disorders. It has a significant effect on physical discomfort symptoms, especially gastrointestinal discomfort symptoms related to anxiety and mood disorders. Analyze and detect the samples of Tianeptine by using eclassical 3200 liquid chromatograph and classic Supersil ODS2 column.
◆Preparation method of test solution:
Accurately weigh 10 mg of thianapeptine to the nearest 0.0001 g, dissolve it in methanol and prepare a 1 mg/ml working solution.
◆Chromatographic conditions and configuration:
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Chromatographic conditions |
Instrument configuration |
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chromatographic column |
Supersil ODS25 μm 4.6x150mm |
Pump |
p3200 high-pressure constant flow pump (1 set)
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mobile phase |
A: Methanol: acetonitrile: dodecyl sulfate = 21:31.5:47.5
B: Methanol: acetonitrile: dodecyl sulfate = 20:60:20 gradient elution
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Detector: |
D3200 UV-Vis detector (1 set)
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Flow rate |
1.0ml/min |
Injector: |
S3200 automatic injector (1 set)
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Detection wavelength |
220nm |
Column Compartment |
03200 chromatographic column temperature box (1 set)
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Injection Volume |
10μL |
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column temperature |
30 ˚ C |
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◆Test solution profile:
The latest EClassical 3200 liquid chromatograph is paired with a classic Supersil ODS2 column to analyze the tianeptine samples with a better resolution, which meets the demand for qualitative and quantitative analysis.
