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Key Technology of Proanthocyanidin Production From Grape Seed Extract

Dec 18, 2018

In 1967, American Joslyn extracted and separated four polyphenols from grape skins and grape seeds, and heated them in an acidic medium to produce cyanidins. Therefore, the polyphenols are named Proanthocyanidin (PC). These compounds are the main functional substances in grape seed extract, have a variety of biological activities, has been widely used in food, medicine, cosmetics and other fields.


Though there are cyaidins in plants, proanthocyanidins is one of the best sources of proanthocyanidins extracted from grape seeds due to its rich resources, and the content of proanthocyanidins in the extract can be up to 95%. At present, the research on grape seed proanthocyanidins is relatively mature.


Proanthocyanidins can significantly improve the body's anti-aging ability, improve cardiovascular function, prevent hypertension, enhance the body's ability to resist mutations, and even have therapeutic effects on arteriosclerosis, gastric ulcer, colorectal cancer, cataract, diabetes, heart disease, arthritis and other diseases


Grape seed is a by-product of wine, accounting for 4% ~ 6% of the whole grape.Proanthocyanidins content in grape seed shell is much higher than that in kernel.At present, the process of extracting proanthocyanidins by degreasing is generally adopted, and the degreasing method will have an effect on the extraction rate and quality of proanthocyanidins.Degreasing methods include press method, solvent method and supercritical C02 extraction method.Press method because of its extraction rate is low, waste is big, do not see very much now.Solvent method is the most commonly used method at present, the required equipment is simple, low cost, and extraction rate is considerable.In addition, supercritical C02 extraction, because there is no interference of light and air, can reduce the degree of polymerization encountered in other extraction methods.Tipsrisukond et al. reported that the antioxidant properties of the extracts obtained by supercritical C02 extraction were much higher than those obtained by traditional methods, and the extracts did not need to be concentrated.However, this method has higher requirements on technology and is not easy to be popularized at present.


After oil separation, organic solvents such as ethanol or acetone are generally used to extract the seed shell, and the obtained shell residue is heated to remove the solvent, which can be recycled. The crude proanthocyanidins can be obtained by filtration and spray drying.

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